Helms Thames, A. Rische, C.H., Krier-Burris, R.A., Cao, Y., Hunt, A.C., Klug, N., Zelenetz, S.R., Burleigh, E.R., Aw, R., Wong, D.A., Shaver, Z.M., Seki, K., Guerrero, L., Fernandez, R., Sharma, S., Bochner, B.S., Jewett, M.C., Scott, E.A. and O'Sullivan, J.A.
Selective Elimination of Mast Cells via Siglec-6-Targeted Nanodelivery of Drug Payload.
Journal of Allergy and Clinical Immunology

Olsen, M.L., Copeland, C.E., Sundberg, C.A., Aw, R., Shaver, Z.M., Rao, G., Swartz, J.R., Karim, A.S. and Jewett, M.C.
Design-driven optimization of low-cost reagent formulations for reproducible and high-yielding cell-free gene expression.
Nature Communications 17(1)

Aw, R., Alam, F., Spice, A. and Polizzi, K.M.
A Pichia pastoris Cell-Free Protein Synthesis Platform.
Pichia Protocols. Methods in Molecular Biology 2697

Wong, D.A.*, Aw, R*., Hulbert, S.W*., Qin, Y., Shaver, Z.M., Myers, K.A., Karim, A.S., DeLisa, M.P. and Jewett, M.C.
A Scalable Cell-Free Manufacturing Platform for Two-Step Bioproduction of Immunogenic Conjugate Vaccines.
ACS Synthetic Biology 14(12)
*Joint first authors

Rezani, R*., Aw, R*., Chan, W., Satish, K., Krüger, A., Kightlinger, W. and Jewett, M.C.
Scalable production of a highly active T7 produced using cell free expression.
Biotechnology Bioeng 122(8)
*Joint first authors

Aw, R. and Polizzi, K.M.
Using design of experiments for improving the sensitivity and dynamic range of an in vitro RNA biosensor.
Biotechnology Progress 41(4)

Bidstrup, E.J., Kyle, H., Bandi, C.K., Owitipana, D.N., Chisti, A., Aw, R., Yang, X., Azadi, P., Jewett, M.C., Wang, L-X., Kightlinger, W. and DeLisa, M.P.
Glycosylation of structured protein domains in cell-free reaction environments.
ACS Central Science 14(6)

Redwood-Sawyerr, Aw, R., Di Blasi, R., Moya-Ramirez, I., Kontrovadi. and Polizzi, K.M.
High-Throughput Spectroscopic Analysis of mRNA Capping Level.
Mammalian Synthetic Systems. Methods in Molecular Biology 2774

Bidstrup, E., Kwon, Y.H., Kim, K., Bandi, C.K., Aw, R., Jewett, M.C. and DeLisa, M.P.
Cell-free systems for the production of glycoproteins.
Glycoproteins. Methods in Molecular Biology 2762

Spice, A.J*., Aw, R*. and Polizzi, K.M.
Cell-free protein synthesis using Pichia pastoris: a practical guide.
Cell-Free Gene Expression edition of Methods in Molecular Biology 2433
*Joint first authors

Aw, R., Ashik, M.R., Islam, A.A.Z.M, Khan, I., Mainuddin, M., Isam, M.A., Ahasan, M.M. and Polizzi, K.M.
Production and purification of an active CRM197 produced in Pichia pastoris and its immunological characterization using a Vi-typhoid antigen vaccine.
Vaccine 39(51)

Mastropietro, G., Aw, R. and Polizzi, K.M.
Expression of proteins in Pichia pastoris.
Methods in Enzymology: Recombinant Protein Expression 660

Aw, R., De Wachter, C., Laukens, B., De Rycke, R., De Bruyne, M., Bell, D., Callewaert, N. and Polizzi K.M.
Knockout of RSN1, TVP18 or CSC1‐2 causes perturbation of Golgi cisternae in Pichia pastoris.
Traffic 22(3)

Aw, R., Spice, A. and Polizzi, K.M.
Methods for expression of recombinant proteins using a Pichia pastoris cell-free system.
Current Protocols in Protein Science 102(1)

Spice, A.J., Aw, R., Bracewell, D.G. and Polizzi, K.M.
Improving the reaction mix of a Pichia pastoris cell-free system using a design of experiments approach to minimise experimental effort.
Synthetic and Systems Biotechnology 5(3)

Spice, A.J., Aw, R., Bracewell, D.G. and Polizzi, K.M.
Synthesis and assembly of Hepatitis B virus-like particles in a Pichia pastoris cell-free system.
Frontiers in Bioengineering and Biotechnology 8

Aw, R. and Polizzi, K.M.
Biosensor-Assisted Engineering of a High Yield Pichia pastoris Cell-Free Protein Synthesis Platform.
Biotechnology and Bioengineering 116(3)

Aw, R., McKay, P.F., Shattock, R.J. and Polizzi, K.M.
A systematic analysis of anti-HIV VRC01 antibody expression in Pichia pastoris through signal peptide optimization.
Protein Expression and Purification 149

Moore, S.J., MacDonald, J.T., Wienecke S., Ishwarbhai, A., Tsipa, A., Aw, R., Kylilis, N., Bell, D., McClymont, D., Jensen, K., Polizzi, K.M., Biedendieck, R. and Freemont, P.S.
Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from non-model bacteria.
Proceedings of the National Academy of Sciences 115(19)

Jonas, F.R.H, Royle, K.E., Aw, R., Stan, G-B. V. and Polizzi, K.M.
Investigating the consequences of asymmetric endoplasmic reticulum inheritance in Saccharomyces cerevisiae under stress using a combination of single cell measurements and mathematical modelling.
Synthetic and Systems Biotechnology 3(1)

Aw, R., Barton, G. and Leak, D.
Insights into the prevalence and underlying causes of clonal variation through transcriptomic analysis in Pichia pastoris.
Applied Microbiology and Biotechnology 101(12)

Aw, R., McKay, P.F., Shattock, R.J., Polizzi, K.M.
Expressing anti-HIV VRC01 antibody using the murine IgG1 secretion signal in Pichia pastoris.
AMB Express 7(1)

Tredwell, G.D.*, Aw, R.*, Edwards-Jones, B., Leak, D.J. and Bundy, J.G.
Rapid screening of unfolded protein response in recombinant Pichia pastoris strains using metabolite profiling.
Journal of Industrial Microbiology and Biotechnology 44(3)
*Joint first authors

Robinson, K.S. and Aw, R.
The Commonalities in Bacterial Effector Inhibition of Apoptosis.
Trends in Microbiology 24(8)

Aw, R. and Polizzi, K.M
Liquid PTVA: A faster and cheaper alternative for generating multi-copy clones in Pichia pastoris.
Microbial Cell Factories 15(1)

Edwards-Jones, B., Aw, R., Barton, G., Tredwell, G., Bundy, J.G. and Leak, D.J.
Translational arrest due to cytoplasmic redox stress delays adaptation to growth on methanol and heterologous protein expression in a typical fed batch culture of Pichia pastoris.
PLoS One 10(3)

Aw, R. and Polizzi, K.M.
Can too many copies spoil the broth?
Microbial Cell Factories 12(1)